We asked several questions to ascertain subjects' fall experiences after the ED visit leading to study enrollment (Table 1).
Finally we acknowledge that while our clinically ascertained subjects were not drawn from a cohort with a single diagnosis such as autism or schizophrenia, they were ascertained from a heterogeneously affected group in whom genetic testing was considered clinically relevant.
For this reason, the approach of reverse genomics/genetics was proposed in presentations by James Lupski and Lauren Weiss, in order to, for example, ascertain subjects with the same CNV and then look for common phenotypic manifestations.
We ascertained subjects with LADA and type 2 diabetes from the LADA China multicenter study (46 hospitals throughout China contributed); type 1 diabetes, defined by WHO criteria (4), from the Type 1 Diabetes Collaboration Net (18 hospitals in Hunan Province); and normal subjects were screened by 75-g oral glucose tolerance test from 5,000 Chinese in a population-based study.
Because of recruitment from the larger SEARCH study, which had ascertained case subjects diagnosed through age 19 years, the oldest of the case subjects had aged to 22 years at the time of recruitment into the SEARCH-CC study.
Ascertained case subjects were contacted and asked to complete an initial patient survey, and subjects completing the initial patient survey were invited for an in-person visit, during which anthropometric and clinical data and samples were collected.
Capture-recapture was not conducted for the membership-based center because the data sources used to ascertain case subjects within the membership-based center were not independent.
We applied the nested case-control design to ascertain study subjects and two control groups from the healthy population, investigating the high-order interaction of risk factors on steatohepatitis.
To determine gastrointestinal (GI) permeability and fecal calprotectin concentration in children 7 to 10 years of age with functional abdominal pain and irritable bowel syndrome (FAP/IBS) versus control subjects and ascertain potential relationships with pain symptoms and stooling.
All patients and control subjects were ascertained in the outpatient ophthalmology clinic at the University of Iowa.
