We repeated this using PfSir2 for immunoprecipitation and probing with PfSUMO and obtained corresponding results (Fig. 5C), thus ascertaining modification of PfSir2.
To ascertain any modification of the effects of GLP-1 following repeated subcutaneous administration, breakfast tests with 0.63 μg/kg GLP-1 were conducted on the day before and on the day after each five-day study.
Oikopleura and Ciona therefore provide us with a model for investigating the role of mechanisms such as co-option and divergence in notochord evolution and for ascertaining the extent of modifications that its genetic toolkit can sustain without major morphogenetic changes.
In the mark-based case it was difficult to ascertain which histone modifications discriminate enhancers from promoters.
Thus, this study aims to collect information about experiences early in the implementation of the initiative in order to ascertain whether program modifications are needed.
Different colours were used as an organizational tool to highlight proposed modifications ascertained from the collected data suggested by the following: participants from their respective community; participants from the other two study communities; and relevant academic literature.
However, to ascertain whether a modification in DNA synthesis occurred selectively in specific cellular stages in the two culture conditions, nuclear spreads were simultaneously immunostained with anti-BrdU and anti-Scp3 antibodies.
To ascertain if SUMO modification is sufficient to inactivate the inhibitory functions of VHL on tumor cells, we tested whether PIASy-induced SUMO1 modification of VHL resulted in dysregulation of VHL as the cells exit the cell cycle on serum deprivation.
Stoichiometric amounts of reduced ERp57 and oxidized ERp72 were incubated together and their redox status ascertained by AMS modification at various time points.
Regimen modification date, one main reason for the regimen modification, and the regimen after modification were ascertained by the cohort database or medical charts.
(C) Expert technical advice should be obtained when analyzing similar items, or commercial items that are "of a type" or requiring minor modifications, to ascertain the magnitude of changes required and to assist in pricing the required changes.
