We used the trypan blue dye exclusion assay to ascertain cell death in Jurkat T cells treated with TPT or TBT as described previously (Chen et al. 2005).
In this study, a high-dimensional image-processing program CalMorph was used on digital microscopic images to ascertain cell shape, actin, and nuclear DNA morphology.
To compare photoreceptors on an ultrastructural level, examples of weaker knock-down with discernible elements of outer segments were chosen to ascertain cell identity (Fig. 4E,F, stars).
Additionally, to ascertain cell death, cells were incubated with 2.5 mg/mL propidium iodide (PI) for 5 min at room temperature and analyzed with a FACSCanto II flow cytometer (Becton Dickson, Franklin Lakes, NJ, USA) and Flowing Software (version 2.5.1; http://www.flowingsoftware.com/).com/
This study has examined cells from naturally-occurring murine mammary tumours to ascertain whether cell surface glycoproteins play a significant role in colonisation of the lungs after intravenous inoculation.
Cell survival following genipin, menadione, doxorubicin, or epirubicin exposure was ascertained using cell viability and Propidium Iodiode (PI) assays.
Immune-gold staining has been widely applied in EM studies to ascertain different cell organelles as well as to conduct 3D reconstruction of cell structure.
The immunocytochemical characterization was also carried out on cells plated at varying densities to validate our qualitative microscopic observation and ascertain whether cell density would have pronounced effect, if any, on neural differentiation.
Some experiments were performed with fibroblasts alone and some by simultaneously seeding fibroblasts and osteoblasts (pre-stained with different fluorescent markers; Molecular Probes) to ascertain competitive cell adhesion.
Thus, there are at least two processes, with different measurable endpoints, that operate at the cellular level to ascertain NK cell tolerance.
