Sentence examples for ascertained using cell from inspiring English sources

Cell survival following genipin, menadione, doxorubicin, or epirubicin exposure was ascertained using cell viability and Propidium Iodiode (PI) assays.

The sensitivity of dPCR was ascertained using cell lines with c-erbB-2 amplification, and the relationship between dPCR ratio value and gene copy number was established.

Staining specificity was ascertained using MCF-7 cell pellets (caspase-3 null) and staurosporine-treated HUVEC cells as negative and positive controls, respectively.

Automated image acquisition using a 2× objective is performed and the area of collagen I and fibronectin per cell is ascertained using the Metamorph® Imaging System software.

The MOI used was 20 viral particles per cell, which was sufficient for infection of >80% of the cell population as ascertained using an adenovirus encoding GFP (data not shown).

That TNF-α preparations used were functionally active was ascertained using human umbilical vein endothelial cells (Huvecs), which were stimulated with 10 ng ml−1 TNF-α.

Equal parts cell suspension and 0.4% trypan blue (Sigma-Aldrich, St . Louis MO, USA) were mixed immediately before cell viability was ascertained using a Bright Line hemocytometer (Reichert, Buffalo, NY, USA) and Nikon TMS phase-contrast microscope (Nikon, Tokyo, Japan).

Long-term effects on cell proliferation were ascertained using the colony formation efficiency (CFE) assay, as described previously.

The safety of the extracts was ascertained using the 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) assay on Vero African green monkey kidney cells.

Thermal stability of the PSMA/TiO2 hybrid was ascertained using TGA[1 10].

Technical acceptability was ascertained using recently-validated optimizations [31].