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The proposed methodology allows for the critical temperature gradient and critical average temperature to be ascertained using an iterative method.
The CHD cases were ascertained using an annual follow-up questionnaire and validated by an End Point Committee through a review of the medical records.
Life events were ascertained using an 11 item questionnaire (derived from [ 29]).
APP was ascertained using an algorithm comprising of two steps, as illustrated by Fig. 1.
Microsatellite alleles were ascertained using an ABI Prism™ 310 automatic sequencer (Applied Biosystems, Foster City, CA, USA).
The MOI used was 20 viral particles per cell, which was sufficient for infection of >80% of the cell population as ascertained using an adenovirus encoding GFP (data not shown).
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The urinary and plasma biomarker performance was ascertained using a receiver operating characteristic (ROC) curve analysis.
As explained in the section describing "Motion classification", the SVM classification performance depends on selection of kernel parameter (upgamma) and penalty parameter C. The optimal combination of (upgamma) and C was ascertained using a grid search within the training data.
Transmission line (TL) backflashover (BF) performance has been traditionally ascertained using a single number, the so-called backflashover rate, which is measured in number of BF events per 100 km-years.
The sequence and minor groove specificity of the achiral compounds was ascertained using a Taq DNA polymerase stop assay and a thermal induced DNA cleavage experiment using either a fragment of pBR322 or pUC18 plasmid DNA.
Using a case control design, MVC deaths were ascertained using a military mortality registry, and an integrated health system database provided information on health system eligibility, pharmacy transactions, and medical encounters.
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