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Job demand and control were not ascertained using a standard questionnaire as these data were collected in 1982 and 1989 when the use of standardised questionnaires was not common.
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The urinary and plasma biomarker performance was ascertained using a receiver operating characteristic (ROC) curve analysis.
β2AR expression was ascertained using a highly quantitative 125I-cyanopindolol (125I-CYP) radioligand binding assay.
Disease history was ascertained using a questionnaire.
Renal function was ascertained using a combination of indicators.
Arterial blood pressure was ascertained using the standard cuff technique with a mercury sphygmomanometer placed on the left arm.
The activity in experimental samples was ascertained using the standard curve of cpm versus the dilution of recombinant IL-2 in the supernatant.
The activity in experimental samples was ascertained using the standard curve of mean absorbance values (OD) versus the dilution of recombinant IFN-γ in the supernatant.
Age, gender, marital status, highest educational level achieved and current living arrangements were ascertained using standard questions.
Complications were ascertained using standard criteria (24).
Macrovascular complications were ascertained using standard procedures.
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