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Because homozygous deletion of Grb2 is not compatible with embryonic viability [ 67*], it was not feasible to ascertain whether Grb2 function was absolutely required for PyV mT tumour progression.
In this study, we examined the underlying mechanism of a CTCM that we used in our previous study in order to ascertain its function in the early-phase response to OVA challenge.
Once candidate genes are identified, functional validation is needed to ascertain the function of the candidate.
DOI: http://dx.doi.org/10.7554/eLife.08378.020 In order to ascertain the function of TFEα/β on productive transcription we fused different promoters to a C-less cassette and carried out transcription assays in the presence of GTP, ATP and UTP.
Further work is now required in order to ascertain the function of all Kirrel3 splice variants in human skeletal muscle and to provide more insight into stimuli promoting its expression and its subsequent post-transcriptional regulation.
The preferred symbol was submitted to NCBI AceView as well as extensive PubMed Boolian logic searches to ascertain the function of the gene in adipose tissue.
To date, traditional phylogenomic studies concentrate on the search for orthology groups in gene family trees to infer the function of unknown proteins, yet the use of such phylogenies to ascertain the function of the encoded proteins is overlooked.
Specifically, the past few years have seen the emergence of CRISPR-Cas9-based strategies to rapidly generate increasingly complex somatic alterations and the development of multiplexed and quantitative approaches to ascertain gene function in vivo.
To determine the optimal glucose concentration, the residual glucose in the culture medium was ascertained as a function of time.
This is the first time that that this assay has been used to ascertain retinal function after cellular therapy in a retinal degenerate animal.
Technicians and engineers inspected all public access water sources to ascertain seasonality, function, and geospatial coordinates.
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