Sentence examples for ascertained the specificity from inspiring English sources

We ascertained the specificity of this binding of the probe to Bapx1 using a supershift assay.

Alkaline-degraded OLO samples were chromatographed to ascertain the specificity of the developed method for OLO.

All ChIP samples were tested for false-positive PCR amplification by sequencing amplicon DNAs to ascertain the specificity of the SALL4 that bound to the cis-regulatory elements.

Immunofluorescence control experiments were conducted to ascertain the specificity of our dystonin-b antibody and to rule out the possibility of non-specific signals.

To better ascertain the specificity of the in vitro recombination reaction catalyzed by IntI1, two mutated enzymes containing amino acid substitutions R146K and R280E were assayed.

Placental DNA samples of the heterozygous C/G, homozygous C/C and homozygous G/G genotypes were tested with the HLCS and rs6636 duplex assay to ascertain the specificity for detecting either one of the SNP alleles.

To ascertain the specificity of doublecortin antibody and cRNA anti sense probe in juvenile and adult human hippocampal tissues we included human fetal (GW 11, 20) brain tissue as positive control and reference for western blotting (Fig. 1), in situ hybridization (Figs. 2), and RT-PCR (Fig S5).

To ascertain the specificity of the new tool, western blot and immunohistochemical control experiments were performed which showed that the antibody could be used to detect endogenous as well as transgenically encoded FKH protein in larval extracts with very little unspecific background (Figure S2A).

The PCR products were also analyzed by melting curve analysis to ascertain the specificity of amplification.

We also assessed NIS expression by immunoblot analysis to ascertain the specificity of the observed immunoreactivity.

To ascertain the specificity of the pattern of staining, we aimed to verify these findings with several independent antibodies.