Sentence examples for ascertained the localization of from inspiring English sources
To verify the relationship between PI4KIIα and the CARTS complex, we ascertained the localization of PI4KIIα, an important component of CARTS (Rab8) and the most classical cargo of CARTS, PAUF, by immunofluorescence.
Besides addressing the issue of where TWIST1 mRNA is predominantly expressed in breast cancer tissues as described above, we also performed IHC staining to ascertain the localization of TWIST1 protein.
Part of the difficulty in ascertaining the localization of GSV/IRV in adipose cells (whether 3T3-L1 preadiporytes or mature primary adipocytes) is their limited cytosolic space, and this challenge is also encountered in mature skeletal muscle fibers.
In order to unambigously ascertain the localization of CaNDR1a, the N-terminally HA-tagged version of CaNDR1a was transiently expressed in tobacco leaves and purified PM fractions were directly tested for the presence of the protein by immunoblotting using HA-specific antisera.
Because of its low abundance, we are unable to ascertain the subcellular localization of Ppt1 and thus could not determine whether fly Ppt1 is lysosomal, synaptic, or both.
To ascertain the subcellular localization of FIG-ROS(S), we performed immunofluorescence assay with 3T3 cells stably transfected with the ROS fusion variants with Myc-tag antibody.
Afterwards, immunofluorescence studies were conducted to ascertain the cellular localization of increased EGFR in cells treated with PC 0.1% in pH 7.0.
We also ascertained the demographics of the study participants.
Together, these results confirm staining of the Golgi apparatus with the anti-PtdIns4 P antibody, although it is not possible to ascertain the sub-Golgi localization of the signal at the resolution of the light microscope.
