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In this analysis we ascertained the expression levels for the first five splice variants in the same reading frame (Fig 3).
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This procedure compared subsequent timepoints with the earliest timepoint, to ascertain whether the expression level of the probeset was either stably (↑ or ↓, Figure 4) or transiently (↑↓ or ↓↑, Figure 4) altered.
To ascertain the observations, the expression level of IL-1 β in BAL cells was analyzed by immunofluorescence staining.
Therefore, Western blot analysis was performed to further ascertain the effect of WHI-P154 on the expression levels of ABCG2.
Furthermore, to ascertain the mechanism by which PFOS reduces BDNF signalling, we examined the expression levels of miR-16 and miR-22, which potentially regulate BDNF mRNA translation at the posttranscriptional level.
To ascertain the tumours that presented changes in the expression of β-catenin, we compared the expression levels of β-catenin before and after CRT.
It was not possible to ascertain clear divisions based on the intratumoral uptake pattern of [F]EF5 according to tumour size, or the expression levels of the studied growth factors.
To ascertain if hypoxia increases gCSC expression of pSTAT3 and HIF-1α, the gCSCs were exposed to hypoxia and normoxic conditions and the expression levels of pSTAT3 and HIF-1α were determined by flow cytometry analysis.
To further ascertain the cellular changes occurring in GD18 LPS-exposed foetal forebrains that might underlie the defect in neuronal patterning, we examined the expression levels and distribution of GLAST, a marker for radial glia.
The expression levels of gene-associated CGI-containing genes and orphan CGI-containing expressed sequence tags (ESTs) were analyzed using RT-qPCR to ascertain the correlation between the aberrant DMRs and the corresponding mRNA expression levels.
We next sought to ascertain the expression pattern of EMT drivers along the EMT Spectrum.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com