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In this analysis we ascertained the expression levels for the first five splice variants in the same reading frame (Fig 3).
As the regulation of SIRT1 has not been investigated in cancer cells lacking ER, in the present study we ascertained the expression and function of SIRT1 by estrogens in ER-negative breast cancer cells and cancer-associated fibroblasts obtained from breast cancer patients.
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We next sought to ascertain the expression pattern of EMT drivers along the EMT Spectrum.
In order to ascertain the expression pattern of the three identified cell-death-related genes in response to Foc inoculation in banana, suspension culture cells of banana (cv.
Methods: RT-PCR was used to ascertain the expression of RNF138 mRNA in the glioma cell lines U251, SHG44, U87, A172, and U373.
Because the incorporation of halogenated nucleotides into DNA could affect gene expression, the fusion experiment involving labeling with halogenated nucleotides is done separately from the fusion experiment for ascertaining the expression status of genes.
To ascertain the expression of activated Notch1 in gastric tumour tissue, we examined the expression of ICN1 in the training and validation sets of specimens and matched the non-tumour specimens by IHC staining.
Using high-quality matches (>85% identity, >100 bp overlap, E-value < 1.0e-15) wereere able to ascertain the expression of 316 orthologous genes (see Additional file 1). Figure 4 shows the quality of the matches.
Because many of our ongoing gene engineering experiments rely on the vasa promoter (Papathanos et al. 2009) for expression of enzymes in the germ cells, we wanted to ascertain the expression pattern of this promoter when inserted at the X1 docking site.
To determine the option values on the loan portfolio, the output of our conceptual framework, we must ascertain the expressions for the put and call options.
Analysis by Western blot (Figure 1B) and immunofluorescence staining (Figure 2A) ascertained the following: a) expression of CA125 in NIH OVCAR-3 cells, b) abseNIH OVCAR-35 expression in SKOV3 cells, and c) binding of MabsencescFv toftarget antigen CA125.
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