The phrase "ascertained sequence" is correct and usable in written English.
It can be used in contexts where you want to refer to a sequence that has been determined or established through investigation or analysis.
Example: "The researchers followed the ascertained sequence of events to understand the cause of the phenomenon."
Alternatives: "determined sequence" or "established sequence".
Once the quality of the fosmids was ascertained, sequences were determined using Roche 454 GS FLX Titanium technology, according to the manufacturer's protocols (Roche Applied Science, Indianapolis).
The PCR genotyping assay allowed us to ascertain sequence similarity between P1-mm and P1-wr.
Accurately ascertaining sequences in these regions with next generation sequencers is challenging, and requires a different set of analytical techniques than for reads originating from unique sequences.
We demonstrate the importance of exploring unmapped reads to ascertain sequences that are either absent or misassembled in the reference assembly and for detecting sequences indicative of parasitic or commensal organisms.
DNA sequencing using T7 promoter (forward, 5´-TAATAC GACTCACTATAGGG-3´) and pJET1.2 (reverse, 5´-AAGAACATCGATTTTCCATGGCAG-´3) sequencing primer ascertained the sequence of the recombinant Hos2 gene.
The identity of these sequences was ascertained by sequence comparisons using the program BlastN of the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/).nih.gov/
The identity of these sequences was ascertained by sequence comparisons using the program BLAST Basic Local Alignment Tooll) of the National Center for Biotechnology Information (http://blast.ncbi.nlm.nih.gov/) By IFAT, four cats/samples tested positive for Giardia duodenalis cysts and one cat for Cryptosporidium sp. oocysts.
The identity of each of these pig defensins was ascertained by sequence comparisons and cysteine positions and spacing.
However, it should be noted that these GO annotations were ascertained by sequence homology rather than direct experimentation so should be taken with caution.
CE would yield the size of an amplicon, i.e., equivalent of repeat length, which can be ascertained from sequence data simply by counting the number of nucleotides within the repeat region.
