The phrase "ascertained following" is not correct and does not convey a clear meaning in written English.
It may be intended to indicate that something was determined or established after a certain point, but the phrasing is awkward and unclear.
Example: "The results were ascertained following the completion of the experiment."
Alternatives: "determined after" or "established subsequent to".
The diagnosis of ICUAW was made on clinical grounds after the patients had been awaken from sedation and their orientation and cooperation had been ascertained, following a protocol described previously [11, 19].
Since then, 267 iCJD cases have been ascertained, following human growth hormone (hGH) injection, dura mater grafts, corneal transplants, neurosurgery, gonadotropin administration, and stereotactic EEG[4].
Melting temperature (Tm) and peak height for each strain was calculated after using SeptiFast® amplification, also adequacy of the values obtained was ascertained following the manufacturer criteria for assigning genera and species identification.
The DAZ deletions were ascertained following standard method [ 28] that showed intactness of the copies of DAZ gene.
The diagnosis of ICUAW was made on clinical grounds after the patients had been awaken from sedation and their orientation and cooperation had been ascertained, following a protocol described previously [ 11, 19].
We also believe that the benefits of surgical treatment of metastatic lesions need to be ascertained by following up the patient over time.
Activity of the protease in the parasite was ascertained by following the degradation of GFP in a transgenic parasite line expressing fusion protein of GFP and Arc-repressor gene, a known target of HslV protease in the prokaryotes.
Analysis by Western blot (Figure 1B) and immunofluorescence staining (Figure 2A) ascertained the following: a) expression of CA125 in NIH OVCAR-3 cells, b) abseNIH OVCAR-35 expression in SKOV3 cells, and c) binding of MabsencescFv toftarget antigen CA125.
At each maternal contact, we ascertained the following information for the infant: the number of household members and siblings, breast-feeding status, cigarette and cooking smoke exposure, symptoms of upper respiratory tract infection, and antibiotic use.
