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The catalytic mechanism of family I.1, which includes LipK107, was ascertained first through comparisons of the sequences and structures of lipases from other families.
The total absence of in vitro toxicity and immunogenicity was ascertained first, and then cell localization studies in cell cultures of fluorescent variants were performed.
The first, consisting of 17 instructions, was written by Kilburn, and so far as can be ascertained first ran on 21 June 1948.
We ascertained first that oleosin gene could be expressed properly in animal cells by subcloning the peanut gene into pSP64 polyA plasmid for in vitro expression in rabbit reticulocyte lysate.
However, whenever dealing with inter-specific neuron homologization across divergent taxa, correspondences between the investigated commissural systems should be ascertained first, before a comparison of the exact course of single contralateral neurite projections makes sense.
Total energy intake and intakes of alcohol, magnesium, calcium, vitamin D, total trans fat, fiber from cereal, and caffeine were ascertained first in 1980 using a semiquantitative food frequency questionnaire (FFQ) and then updated every 2 4 years from subsequent FFQs.
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We identified patients and obtained treatment details from 12 cancer centres (11 United Kingdom, 1 Norway) and ascertained second cancers and mortality through national registries.
I made a visit to Haiti last weekend to meet with the President of the Haiti FF to ascertain first hand the needs of our football family.
To ascertain first-time use, we required that the patients had at least 180 days of prescription history for any prescribed drug before the index date.
55 Our main strengths include the use of data from the entire population of approximately 23 million individuals, and systematic methods to identify and ascertain first-degree and second-degree relatives, which allow very precise estimation of prevalence and RRs of gout with minimal selection bias.
The reliability of the questionnaire was ascertained by first conducting a pre-test of the questionnaire.
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