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The parameters of the controller are ascertained by using a genetic algorithm.
Exposure assessment was ascertained by using a geographic information system to link the study subjects to specific water supplies.
By excluding individuals residing in assisted living facilities, we minimized the possibility of recruiting older women with significant cognitive impairment, as further ascertained by using a dementia screener.
Familial recurrence of melanoma was ascertained by using a questionnaire to interview probands about their first-, second-, and third-degree relatives.
Approximate timing of infection for patients with IgG-positive samples was ascertained by using a commercial solid-phase enzyme immunoassay (Toxoplasma gondii IgG Avidity EIA; Ani Labsystems Ltd. Oy, Vantaa, Finland) to determine avidity (binding ability) index.
The presence of this species was ascertained by using a 5S 23S spacer amplicon after digestion with MseI and demonstration of fragments having sizes typical for B. spielmanii (106, 68, and 51 bp) (2 ).
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Longitudinal stability in the measurements over years was ascertained by using an elaborate system of internal quality control samples and testing of incoming reagents (Appendix A).
Lack of genomic and foreign DNA contamination was ascertained by using all RNA samples and water as a template in a PCR.
Lack of genomic and foreign DNA contamination was ascertained by using all RNA samples and water as a template in a PCR reaction.
The density of the preform was ascertained by using Archimedes's principle at room temperature with an accuracy of ±1 %.
The enzyme activity was ascertained by using cumene hydroperoxide (both selenium and nonselenium enzyme types) as a substrate and GR coupled assay to monitor the oxidation of GSH [ 41].
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