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The SNPs were all identically ascertained by microarray resequencing of the genome, and verified in multiple populations.
In order to ascertain whether or not data obtained by microarray analysis with low-RIN samples were comparable to the results obtained using the method designed by Antonov et al for qPCR analysis of low-RIN samples, we selected two genes, dipeptidase 1 (DPEP1) and claudin 1 (CLDN1), for qRT-PCR validation.
Many of the additional genes encode regulatory proteins, whose altered transcription may be difficult to detect by microarray analysis, and whose regulatory control may be difficult to ascertain in the absence of experimental data.
A) Expression revealed by microarray analysis.
Relative expression levels were determined by microarray analysis.
Open image in new window Figure 1 Identification of ISG candidates by microarray analysis.
RBM24, which has been shown to be up-regulated by HCV infection by microarray analysis, was selected for further study.
We found that DR6 was highly expressed in human pDCs comparing with other blood cells by microarray analysis (Fig. 1A).
Overall, 667 miRNAs were determined in serum pools of TB patients and healthy controls by microarray analysis.
The potential presence of false positives and false negatives in a microarray experiment requires validating a subset of the information (genes) identified by microarray analysis.
To validate the results from the microarray, real time qRT-PCR was performed on 12 genes detected by microarray analysis.
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