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The phrase "ascertained by increased" is not correct and sounds awkward in written English.
It may be intended to convey the idea of determining something through an increase, but it lacks clarity and proper structure.
Example: "The results were ascertained by increased data collection over the past year."
Alternatives: "determined through increased" or "established by heightened".
Exact(1)
SIM+MET treatment led to enhanced autophagic flux in C4-2B cells by 72 96 h, ascertained by increased LC3B-II (further enhanced with lysosomal inhibitor chloroquine) and reduced Sequestosome-1 protein expression, significantly increased percentage of acidic vesicular organelle-positive cells, and increased autophagic structure accumulation assessed by transmission electron microscopy.
Similar(59)
Generation of hypertrophy on the other hand, was ascertained by significant increase in the heart weight to body weight ratio, increased cardiomyocyte crosssectional area and significant increase in the expression of hypertrophy marker genes atrial natriuretic factor (ANF) and β- myosin heavy chain (β- MHC) compared with respective controls, both in vivo as well as in vitro as shown earlier.
Lesion volume, ascertained by TTC staining, increased with longer durations of hypoxia.
DBM allografts seem to be more favorable with respect to cell ingrowth tested by histology, and osteogenic differentiation ascertained by an increase of OC.
This was ascertained by an increase in height velocity of at least 50%% during the first prepubertal year of GH treatment (33 μg/kg/d).
This is ascertained by the increase in transcripts encoding (I) metabolic-related enzymes such as CYP450 and COX, (II) sugar transports such as HXT2 and H+-ATPase and (III) cellular response such as Hsp70 and Hsp90.
EGFR signaling was increased in cells with diminished VDR expression as ascertained by higher EGFR, phosphorylated EGFR, and phosphorylated ERK protein levels (Fig. 7A).
Apparently, the addition of gemcitabine did not increase DXR activity, although this could only be ascertained by a comparative study.
Second, from a horizontal perspective, the significance of public and private kindergartens can be ascertained by their numbers: the number of public kindergartens has decreased sharply since 1980s, while that of private kindergartens increased substantially as a supplement.
This has been ascertained by measurements at several temperatures above the ambient and the resulting capacitance decreases as temperatures increases.
Drug changes (i.e., new prescriptions or increased dose of a previously used drug) were ascertained using the computerized provider order entry system, whereas falls were ascertained by incident reports.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com