Cardiovascular complications were ascertained using standard procedures.
The urinary and plasma biomarker performance was ascertained using a receiver operating characteristic (ROC) curve analysis.
As explained in the section describing "Motion classification", the SVM classification performance depends on selection of kernel parameter (upgamma) and penalty parameter C. The optimal combination of (upgamma) and C was ascertained using a grid search within the training data.
Transmission line (TL) backflashover (BF) performance has been traditionally ascertained using a single number, the so-called backflashover rate, which is measured in number of BF events per 100 km-years.
The sequence and minor groove specificity of the achiral compounds was ascertained using a Taq DNA polymerase stop assay and a thermal induced DNA cleavage experiment using either a fragment of pBR322 or pUC18 plasmid DNA.
Using a case control design, MVC deaths were ascertained using a military mortality registry, and an integrated health system database provided information on health system eligibility, pharmacy transactions, and medical encounters.
β2AR expression was ascertained using a highly quantitative 125I-cyanopindolol (125I-CYP) radioligand binding assay.
Proper placement of the epidural catheter was ascertained using a 3-mL test dose of lidocaine 1% to 2% with epinephrine 1∶200000.
Equal parts cell suspension and 0.4% trypan blue (Sigma-Aldrich, St . Louis MO, USA) were mixed immediately before cell viability was ascertained using a Bright Line hemocytometer (Reichert, Buffalo, NY, USA) and Nikon TMS phase-contrast microscope (Nikon, Tokyo, Japan).
To test if obtaining accurate chromosomal haplotypes can be achieved using high-density SNP arrays and if this would provide more complete segregation information, we directly compared high-density SNP genotype analysis with experimentally determined haplotypes ascertained using a panel of haploid mouse-human hybrid cell lines generated from ALS-A family members [20].
