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The phrase "ascertain the substrate" is correct and usable in written English.
It can be used in contexts where you need to determine or identify the underlying material or foundation of something, often in scientific or technical discussions.
Example: "In order to conduct the experiment accurately, we must first ascertain the substrate on which the reaction will take place."
Alternatives: "determine the substrate" or "identify the substrate".
Exact(1)
However, the annotation is not precise enough to ascertain the substrate molecule class.
Similar(59)
To ascertain the role of the substrate, the expression profile of cells cultured on plastic plates was also compared with that of cells grown on collagen I in basal conditions; we refer to this experiment as "coll-vs-plast".
The PKCIW in situ signal was revealed first using Tyramide fluorescent substrate (Methods) to ascertain the sex of the embryo.
*blotting with mAb 4G10 anti-PTyr was used to ascertain the level of tyrosine phosphorylation after immunoprecipitation with substrate-specific Abs. a statistically equal level of relative substrate phosphorylation between FAK+/+[Src] and FAK-/-[Src] cells, based on triplicate experiments.
In this study, the variation in mechanical and electrical properties was characterized in engineered porous metal foams with aligned porosity to ascertain the degree of anisotropy that can be induced in these substrates.
To further ascertain the formation of eccDNA in vitro we performed the reaction using artificial substrate as a template DNA.
The specific substrates remained to be found for CYP2C93, which can partly ascertain the relevance of this enzyme to drug metabolism.
Control reactions (heat inactivated enzyme and substrate control) were set up in a similar manner to identify and ascertain the product formation to be through the enzymatic reaction catalyzed by the SGT (GsSGT).
To ascertain the physiological role of the IclR family of regulators in the cell, identification of the interaction between the ligand and the substrate-recognition domain of the regulator would provide valuable insight.
In order to ascertain the commercial application and performance of the developed PS-FLG material, we fabricated a symmetric solid-state device (SSD) by coating the synthesized PS-FLG onto a conducting substrate (ITO) and using PVA-H2SO4 as the gel electrolyte (Fig. 8c, inset and Fig. S11).
Charlie Flanagan said the government was trying to "ascertain the facts".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com