We wanted to ascertain the sequencing of DTP and concept uses: we made these distinctions explicit by breaking down the overall visit time for each clinician into four segments of equal length, thereby suggesting a first contact and initial gathering of information in the first 25% of the visit time, and closure and presentation of treatment plan in the last 25% of the visit time.
DNA sequencing using T7 promoter (forward, 5´-TAATAC GACTCACTATAGGG-3´) and pJET1.2 (reverse, 5´-AAGAACATCGATTTTCCATGGCAG-´3) sequencing primer ascertained the sequence of the recombinant Hos2 gene.
To ascertain the sequence similarity of A. mongolicus to other plant species, total unigenes were searched by BLASTN against the PlantGDB-generated Unique Transcripts (http://www.plantgdb.org/prj/ESTCluster/) (similarity ≥ 80% and E-value ≤ 1e-5).
We used for each alignment position a Wilcoxon-Mann-Whitney two-sample rank sum test to ascertain whether the sequences of one set have on average a different probability of base pairing at that position with respect to another set.
For a candidate task set c, we need to find the set of acceptable tasks ( A=left{{j}_1^{prime },{j}_2^{prime },dots, {j}_{left|Aright|}^{prime}right} ), ascertain the valid sequence of A, and then maximize the total profit.
First, the study design was a retrospective cross-sectional analysis, for which it is usually difficult to ascertain the temporal sequence of exposure and outcome.
It is well established that the ultimate goal in DNA research is to ascertain the DNA sequence of a gene.
Further studies are required to ascertain the exact sequence of time-dependent (radio biological effects during neoadjuvant multimodality treatment.
However, detection of such associations is far from establishing causality since such studies are unable to ascertain the temporal sequence of exposure and outcome (LaKind et al., 2008b, 2012).
Finally, in this initial set of experiments, MV4-11 cells were treated with the CNDAC/vorinostat combination using the three treatment schedules evaluated in the cytotoxicity experiments to ascertain whether the sequence of compound administration had a significant influence on the amount of cell death.
