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The entire lymph node and ankle tissues were digested in 1800uL of the buffer, 80 uL of which was analyzed by flow cytometry to ascertain the cell population distribution in the lysate.
These results could also have implications in the studies that analyze chemokines or chemokine receptors expression in melanomas that do not ascertain the cell membrane location of chemokine receptors or the secretion of chemokines to the extracellular medium.
Because other cell populations can produce these cytokines and we observe both up-regulation of IL-4 and down-regulation of IL-10, which can both be produced by TH2 cells further study is necessary to ascertain the cell populations affected by iHg.
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The official acknowledged that most scientists were aware of perhaps only a dozen lines in the United States, but said the N.I.H. deployed its researchers to ascertain the cells available in private labs throughout the world.
To investigate cell death, we performed propidium iodide staining of fixed cells to ascertain the percentage of cells in the sub-G1 population.
Western blot experiments were performed to ascertain the levels of cell cycle regulatory proteins in MIAPaCa cells, which contain p53 gene mutations (data not shown).
To ascertain the timecourse of cell recruitment to the fin injury area, a measure of cell density near the resected fin edge was utilized.
Annexin-V exposure, even though considered as an early event of apoptosis, had been initially characterized in necrotic cells.9 Likewise, it is well established that most apoptotic cells later shift to necrotic status owing to membrane permeability.10,11 This makes it very difficult to ascertain the nature of cell death signaling from snapshot events of analysis using this assay.
Apoptosis studies were carried out to ascertain the mode of cell death.
To ascertain the efficiency in cell separation and the lymphatic nature of HL-LECs, FACS analysis of the expression of Pdn was performed.
To ascertain the dosage for cell treatment, we carried out a detail drug release study using 4 mg of sample loaded with DOX under various thermal conditions.
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