Sentence examples for ascertain that only from inspiring English sources

Each primer pair was evaluated for specificity by melting curve analysis to ascertain that only one product was amplified and a BLAST search was performed to confirm that primer sequences amplified only the target gene of interest.

Melt curve analysis of all samples was routinely performed to ascertain that only the expected products had been generated.

To ascertain that only the 2 1 PhoP–WB6 complex exists, we ran the size-exclusion column with an excess of either DNA or PhoP in the mixture.

The melting peak of each sample was routinely determined by melting curve analysis to ascertain that only the expected products had been generated.

The melting peaks of all samples were routinely determined by melting curve analysis in order to ascertain that only the expected products had been generated.

However, it is often difficult to assess the precise locus and extent of damage in such patients and to ascertain that only the hippocampus and no other medial temporal regions are affected.

At the inquest yesterday, the damaging fact was ascertained that only two of the cowboys were armed, it thus being a most unequal fight".

For Hepatitis B it was ascertained that only 4.9% (6/121) of affected professionals were monitored for one year after the occurrence of exposure to BM; 2.5% (03/121) for Hepatitis C; and 5.8% (07/121) for HIV.

In a blinded experiment on progeny of a single heterozygous F2 plant (C298Δ/WT), we ascertained that only F2 3 progeny plants homozygous for the SACPD-C mutant allele formed aberrant nodules (Additional file 7).

MCMC convergence among the runs was monitored using Tracer 1.5 [ 51], where we ascertained that only four of the twelve runs converged on the shortest trees (only two runs converged for the four-gene analysis; see [ 52- 54]), and the first 40001 trees were to be discarded as burn-in.

Consistent with this evidence, we ascertained that only groups receiving EEN, MNZ, or a combination of EEN and MNZ had reductions in H. trogontum load as well as MIP-2, LIX, and TNF- α (key proinflammatory immunological mediators that contribute to tissue injury in the pathogenesis of the disease) mRNA levels in the colonic mucosa.