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We used MDA-MB-231 and MDA-MB-468 TNBC cells to ascertain interaction between Noscapine (sub IC50 concentration) and Doxorubicin using isobolographic method.
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Last, because the ACCORD trial was not designed to ascertain interactions between CAN and a rapid lowering of A1C or/and hypoglycemia and considering the post hoc nature of our analysis, we cannot draw more specific conclusions regarding the precise mechanisms involved by which CAN increases mortality.
To ascertain interactions, the gap repair technique was performed as in [ 54].
Subsequently, all variables reaching a p value < 0.10 were included in multivariate models to assess independent associations and better ascertain interactions between variables.
We were also interested in ascertaining any interaction between the early manipulation (EHLP) and the post-weaning manipulation (individual-housing) on anxiety-related behavior in the zero-maze.
To ascertain whether interactions between these positive charge sidechains in the non-annular lipid-binding site and the lipid headgroup could affect channel activity, single-channel current recordings were taken of KcsA wild type and mutants reconstituted in a planar bilayer composed of 70% POPC and 30% POPG (Fig. 5).
A two-way analysis of variance was used to ascertain any interaction effects between our primary variables.
To ascertain that the interaction between WRN and p300 was not mediated by DNA, WRN and p300 were co-immunoprecipitated from HeLa nuclear extracts pre-treated with or without ethidium bromide (EtBr), which disrupts non-specific DNA−protein interactions by intercalating and unwinding the DNA helix (Fig. 5C).
Next, we also carried out in vitro solid-phase interaction studies using recombinant PfClpY and PfClpQ to ascertain their interaction.
MPID also allows users to input a group of proteins to ascertain the interactions among them.
To ascertain whether the lack of interaction between MP and P8, RdRp or Pro domains was not due to the lack of expression of the proteins in the yeast cells, the expression of all the proteins, both from pGAD T7 and pGBK T7 clones was monitored using HA polyclonal and cMyc monoclonal antibodies respectively using the lysed transformed AH109 cells grown in -Leu -Trp -His medium.
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