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We took advantage of a qRT-PCR strategy, as it is the most sensitive method to ascertain gene expression levels.
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Here, we assessed the hypothesis that early inflammatory genes are related to arthrofibrosis by ascertaining gene expression during the early stages of contracture genesis in an animal model.
This allowed the authors to ascertain gene-expression profiles for stemness, and to use them as the basis of a scoring system for risk.
We currently do not have data to ascertain whether gene expression level differ between these genomic regions.
It is also possible to ascertain whether variances of gene expression levels follow these evolutionary relationships [ 18].
In both of these instances a quantitative PCR approach seeking to ascertain differences in opsin gene expression levels could help clarify these discrepancies.
EMT gene expression levels were then ascertained in BxPC3 cells treated or untreated (control) with TGFβ1, S100A8, S100A9, and the S100A8/A9 complex and NT-S100A8.
Two different methods were used to ascertain copy-number increases and decreases: threshold analysis and Bayesian Analysis of Gene Expression Levels (BAGEL).
Gene expression levels were normalized using the β-glucuronidase (GUSB) gene as an internal control.
Once we had identified eQTL and eQTMs, we ascertained to what extent SNPs and DNA methylation could jointly explain the variation in liver gene expression levels.
To ascertain whether the differences in yield between strains were due to secretory stress, RT-qPCR was used to analyse gene expression levels of UPR related genes.
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