Sentence examples for ascertain expression from inspiring English sources

In conclusion, we have utilized the most common ADRB2 haplotypes (allele frequency of 0.05 or greater) to ascertain expression and agonist-promoted downregulation.

Since transduction at low MOIs did not result in a substantial decrease of the EGFP expression, Jukat cells were infected at MOI 1 with VSV-G pseudotyped lentiviral vectors encoding both EGFP alone (EGFP) or anti-CEA CIR and EGFP (αCEA-CIR-EGFP), to ascertain expression of a single antibody species per infected cell.

The central goal of this study was to ascertain expression levels for any given transcript across all in vivo models and with successive passage.

The ability to ascertain expression of known and novel SNP and to detect imbalanced allelic expression, in addition to discrete quantification of genome-wide transcript abundance, give RNA-seq of individual embryos enormous utility in studying early developmental processes.

Additional work in ascertaining expression patterns for other commonly prescribed chemotherapy regimens, like anthracyclines and capecitibine, is underway, in the hop that these patterns differ between regimens so that predictive tests for the selection of appropriate treatment can be conducted to minimise toxicity and maximise efficacy for women with breast cancer.

In order to ascertain mRNA expression, RT-PCR for GluR1, 2 and 4 mRNA expression was performed using 2 primary GBM lines and the established U87-MG GBM cell line (Figure 3A).

To ascertain the expression of activated Notch1 in gastric tumour tissue, we examined the expression of ICN1 in the training and validation sets of specimens and matched the non-tumour specimens by IHC staining.

The example genes we chose contained a well-known meiosis gene as a positive control, mitochondrial-encoded genes which have been found to be of interest in this study, and genes expressed at low levels in order to ascertain their expression pattern.

We took advantage of a qRT-PCR strategy, as it is the most sensitive method to ascertain gene expression levels.

To ascertain EpCAM expression patterns, whole embryos were stained with a β-galactosidase staining kit (Invitrogen) in accordance with the manufacturer's instructions.

This design allowed us to ascertain Arc expression in new neurons throughout their proliferative (24 h) phase to their long-term survival (4 weeks) (Fig. 1).