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Cycling conditions consisted of an initial denaturation period of 3 min at 95°C, followed by 40 cycles of 95°C for 30 sec, 62°C for 30 sec and 72°C for 30 sec and a final elongation of 72°C for 10 min. Sequencing products were analyzed by electrophoresis using a 1.5% agarose gel to ascertain correct fragment size, concentration and purity.
Their records were reviewed on inclusion and re-reviewed at least 6 months later in order to ascertain correct classification of the AU [ 2].
The needles were inserted through the spinal cord parenchyma to reach the ventral aspect of the vertebral canal and this position was ascertained using fluoroscopy and observing cerebrospinal fluid dripping from the needle hub (and, in some cases, injections of radiographic contrast agent were used to ascertain correct positioning within the vertebral canal).
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Multilevel linear regression was used for modeling, considering the hierarchical structures of the data aggregation levels, individual VIGITEL variables and contextual variables for the CA to avoid an underestimation of the standard error values, ascertain the correct confidence intervals and correct the hypothesis testing.
Fighters were killed on both sides, according to the two accounts, but it was impossible to ascertain the correct toll.
Personnel are urged to open lines of communications as early as possible with the appropriate authorities to ascertain the correct requirements for any ground support equipment that might be required.
Swedroe again used Morningstar data to ascertain the correct benchmark.
Staff attempted to contact participants directly by telephone or email to ascertain the correct weight.
To ascertain the correct molecular weight of 16.8 kDa, in vitro-transcribed and -translated (IVTT) recombinant survivin protein was loaded.
Participants were encouraged to get the box of the medication in order to ascertain the correct name of the drug.
The ability of GEP to ascertain the correct diagnosis of each biopsy was assessed via diagonal linear discriminant analysis (DLDA) with classification error rates in the training set determined by leave one out cross-validation (LOOCV).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com