Sentence examples for asc i from inspiring English sources

Parenting him has been the deepest and most rewarding education for me, and like many parents of children with an autistic spectrum condition (ASC), I read a lot about the subject, work written by "neurotypical" (a less loaded term for "normal") writers as well as by people on the spectrum themselves.

Finally, an ∼660 bp Asc I fragment containing the Rab5N142I cDNA was ligated into pRO [59].

Simultaneously, the pPCSII plasmid was digested with Asc I and Spe I enzymes.

pMIR-REPORT-NoMp-Insert was digested using Asc I and Pac I.

As circular chromosomes are known to not enter pulsed-field gels [42], we performed in-plug digestion of the chromosomes with Asc I to cleave the single Asc I site present in chromosome V prior to PFGE analysis.

To digest the DNA with Asc I (New England Biolabs) prior to PFGE, plugs were extensively washed in digestion buffer and incubated with 60 units of Asc I in 500 µl of 1X digestion buffer at 37C for 18 h.

A 1.4-kb Not I-Xho I fragment and a 6.6-kb Asc I-Pac I fragment were inserted as a short and long arm, respectively.

The fragments were cloned into pFGC5941 after Asc I-Swa I digestion.

The digested DNA samples were then ligated into Asc I- Not I digested p14 and p17+ plasmid vectors respectively and transformed into E. coli DH10B cells.

The vector pUC19 (Yanisch-Perron et al., 1985) was cut with Hind III and Aat II and the 2170 bp fragment containing the β-lactamase gene and the ColE1 replication origin was isolated, and two different synthetic double stranded oligonucleotides were inserted (order of restriction sites Hind III-Asc I-EcoR I-Xho I-Sfi I/Bgl I-T7promoter-Aat II).

pFlox plasmid was digested with Sal I/Asc I and the resulting fragment was microinjected into the CD2F1 hybrid mice (Balb/C x DBA2).