Sentence examples for asc I from inspiring English sources

To express PUF7-TAP, pHD918 [15] was modified by addition of a polylinker (Avr I– Asc I– Xma I– Sph I) to give pHD1744.

Each linker-adapted DNA sample was then digested with Asc I and Not I enzymes (NEB) and purified using Qiaquick (Qiagen).

Following digestion with Asc I and Not I enzymes (NEB, Pickering, ON), the ds-cDNA was directionally ligated into pUC18-derived vectors, p17+ for the production of driver DNA and p14 for the production of tester RNA.

The double stranded cDNA was purified by phenol chloroform extraction then digested with Asc I and Not I restriction enzymes and ligated into pCDC1-K cloning ready vectors according Epicentre Biotechnologies' protocol.

After 3-rounds of STAR (Fig. 1), the remaining tester RNA was converted to double-stranded DNA, digested with Asc I and Not I and ligated into a similarly digested pUC-modified vector.

When I first came to ASC, I made a purely economic argument for an environmental project.

A 1.4-kb Not I-Xho I fragment and a 6.6-kb Asc I-Pac I fragment were inserted as a short and long arm, respectively.

The fragments were cloned into pFGC5941 after Asc I-Swa I digestion.

The digested DNA samples were then ligated into Asc I- Not I digested p14 and p17+ plasmid vectors respectively and transformed into E. coli DH10B cells.

The vector pUC19 (Yanisch-Perron et al., 1985) was cut with Hind III and Aat II and the 2170 bp fragment containing the β-lactamase gene and the ColE1 replication origin was isolated, and two different synthetic double stranded oligonucleotides were inserted (order of restriction sites Hind III-Asc I-EcoR I-Xho I-Sfi I/Bgl I-T7promoter-Aat II).

pFlox plasmid was digested with Sal I/Asc I and the resulting fragment was microinjected into the CD2F1 hybrid mice (Balb/C x DBA2).