Using the Basic Local Alignment Search Tool (BLAST) as part of annotation and functional prediction, several characterised as well as uncharacterized transcription factors and genes were found in the dataset.
The mother and both daughters carry a premature stop codon in ryanodine receptor subtype 1 (RYR1) as well an uncharacterized RYR1 variant inherited from the father.
Other predicted substrates imply nascent areas of mTOR biology, including vesicle trafficking and endocytosis via the Rab effector Micall1, the Rab guanine nucleotide exchange factor Dennd4C, and the Rab binding protein Plekhm1, as well as uncharacterized sites on a-Raf and c-Raf, which may point to crosstalk between mTOR and MAPK signaling pathways.
Thus, transcriptomic analysis of seeds imbibed at high temperature suggests that select known ABA signaling genes as well as uncharacterized members of the PYL/RCAR, PP2C and SnRK families are differentially regulated during germination at supraoptimal temperature and may play specific roles during thermoinhibition.
Among the other most conserved nematode-specific clusters were homologs of previously characterized C. elegans structural proteins (for example cluster, AAC01973 matched to a collagen family protein, COL-176) as well as uncharacterized C. elegans hypothetical proteins (for example, cluster, AAC01948 matched C. elegans gene, C34E7.4 which has no known function).
Using microarray and in situ hybridization datasets we have produced a model of this network which contains over 600 genes, including numerous retinal disease loci as well as previously uncharacterized photoreceptor transcription factors.
There are several predicted Zinc Finger proteins, a few tripartite motif-containing proteins, as well as some uncharacterized putative proteins.
According to RDP classification, unclassified Acidobacteria or Proteobacteria, as well as other uncharacterized bacteria, were quite common within all sites.
Well-known, as well as previously uncharacterized infections continue to (re)emerge around the globe.
The other LRRs that showed evidence for positive selection included several more characterized LRRs (APL1B, APL1A, and LRIM1) as well as three uncharacterized LRRs (LRR 7030), LRR 7059), LRR 7060)).
