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SPiDER enables a programmer to dynamically change data distributions as well as array values.
It includes a comprehensive discussion of the principles of air gun seismic sources as well as array forming and tuning, and near-/far-field signatures.
Target labeling, as well as array hybridization, washing and staining were performed as described in the GeneChip Whole Transcript (WT) Sense Target Labeling manual (http://www.affymetrix.com).
Pearson's product moment and Spearman's rank correlations were computed using analytical tools integrated into GeneNetwork and using data sets of numerous BXD behavioral and physiological phenotypes, as well as array data of brain gene expression.
cDNA synthesis, cRNA synthesis and labelling, as well as array hybridization were performed as described [ 54].
Messenger RNA extraction, cDNA synthesis and labeling, as well as array hybridization to Affymetrix Yeast Genome S98 arrays, were performed as described in the Affymetrix users' manual [ 48].
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Particular attention is paid to the design of and operation of both silicon and high-speed-gated InGaAs/InP detection systems, as well as array-based systems.
We compare the flexure of single barbs as well as arrays of barbs and find that the interlocking adherence of barbs to one another enables a more robust structure due to minimized barb rotation during deflection.
These include comprehensive but expensive next-generation sequencing-based approaches (i.e.: WGBS [ 6, 7], RRBS [ 8], MethylCap-seq [ 9] or MBD-seq [ 10] as well as array- and PCR-based methods, more affordable and still used [ 11, 12].
Because TA is associated with other congenital anomalies, it is important to perform standard karyotyping as well as array-based comparative genomic hybridization (array-CGH) to detect microdeletions or duplications in the genome.
Judging from comparisons of array-specific MA-plots (results not shown) as well as array-averaged plots of expression ratio versus signal intensity (supplementary fig. 1, Supplementary Material online), we believe that the PTL normalization and normexp background correction is a better treatment of these data than VSN for removing dye bias and other artifacts present in the data.
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