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For estimating duration of G2 and M phases, sections from embryos exposed to EdU for 1, 2 or 3 h were immunostained against pH3 to identify mitotic progenitors and EdU visualized as before.
Bone metastases were visualized as early as day 8 by MRI (T1-Gd-DTPA) before pain detection.
Right panel - HeLa cells depleted of TANGO1 protein by CRISPR procedure were transfected with Mit-TEER-Myc as before and visualized following DAPI staining (blue) and immunostaining for Myc (green) and ERGIC-53 (red).
Colonies were stained and visualized as above.
Alignments were visualized as above.
His eyes would turn to slits above his freckled cheeks as he visualized his next ride.
The samples were incubated with C-labeled chloramphenicol and acetyl-CoA and the radioactive products were separated by thin-layer chromatography silica gel (Merck, USA) and visualized by autoradiography as described before [15].
As before, the reconstructed tracts were visualized in 3D together with the group-averaged fMRI results (Fig. 5).
Murray suggests that researchers begin by using techniques such as immunofluorescence to visualize a cell's organization before deciding whether to break it open.
Cells were identically processed as before for microscopy and visualized in an Olympus TIRF microscope (Olympus IX inverted microscope, 100× PL FL TIRF oil lenses (N.A. 1.45), Photometrics Cascade II 1024×1024 EMCC camera).
The ability of the domains to activate these kinase(s) was tested as described before, using histone phosphorylation visualized by autoradiography [9] (Figure 3A).
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