The lower activity of the kdr ZFP2 zinc finger array in the B2H system may be due to low DNA-binding specificity as well as to the lower stringency of the bacterial one-hybrid (B1H) system used to select the kdr-targeted zinc finger arrays (see Discussion below).
Firms were asked, among other things, to assess the intensity of the demand decrease for their products as well as the stringency of the financial constraints they suffered because of the crisis.
We used the DAVID analysis tools default settings to select the annotation categories that were examined in this analysis as well as the stringency levels utilized by the analysis tools.
The ability to map reads with no errors is relatively trivial and differs between methods due mainly to the upper limit on the number CALs generated before a read is ignored as well as the stringency of post-alignment filters.
If sequence reads are mapped with respect to a reference sequence (such as the rCRS [83]), the particular reads that are mapped or discarded will depend upon the underlying algorithm of the chosen assembler/software program as well as the stringency of the base mismatch and length similarity parameters applied (assuming these latter two features can be adjusted with the selected assembler).
Given the differences in the methodology used to select patients in the two cohorts (90% FISH in Bordet, 88% IHC in the current study), as well as the stringency applied (expanded access cohort with mandatory central confirmation of HER2 status in Bordet, routine clinical care in the current study), it is perhaps not so surprising that we might observe this difference.
RAP shows a remarkable agreement with these validations (dataset name in RAP: " Mouse cerebral cortex adult VS embryonic") with the only exceptions of Igf2bp1 and Mobp, both marked as non significant due to the stringency of Cuffdiff2 algorithm, although both noticeably differentially expressed.
Perhaps countries should pay as much attention to the quality of their environmental legislation as to its stringency.* "The impacts of environmental regulations on competitiveness".
For statistical evaluation, different levels of stringency were applied so as to reflect stringency levels commonly found in the literature [8], [14], [16].
The number of cycles depends mainly on the affinity of the aptamer-target interactions as well as on the stringency imposed to each round of selection.
