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This critical decision influenced the research methods and, particularly in a nonunion environment such as this, necessitated community involvement.
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This necessitated manual background subtraction as implemented in the Excel template used for LRE quantification (additional file 2).
Our objective was to investigate changes in total protein as a potential new biomarker of aging, but this necessitated first the development of a fluorescence microscopy method to detect and quantify any changes.
In addition to rare-earth metal cations, Fe(II), Cu II), Zn II), Cd II), and Pb II) were found to function as efficient and promising Lewis acids in aqueous media, and this necessitated the establishment of the criteria for catalytic activity of the metal cations.
Accordingly, as trade unfolded, imbalances in trade (imports and exports) arose and this necessitated that gold be transferred between nations (in boats) to fund these imbalances.
For the purposes of the present study it was necessary to obtain wide anatomical coverage of both limbs at thigh and calf level in a practical examination time: this necessitated certain compromises in the acquisition design, such as precluding the use of a Carr-Purcell-Meiboom-Gill multi-echo T2-measurement sequence.
This necessitated installing air conditioning, generators, electric cables and toilets.
This necessitated greater standardization of parts and job processes because of the complexity of the product.
Doing this necessitated that women accept a patriarchal definition of leadership and success.
This necessitated alliances with Russia and the Netherlands and, from time to time, France.
"This necessitated an intensive search for new capital that in current market conditions has proved impossible to raise.
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CEO of Professional Science Editing for Scientists @ prosciediting.com