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Out of a search space of 2 × 1012 sequences, the calculation selects a point mutant of the native protein as the top solution (experimental Kd = 17 μM) and the native protein as the second best solution (experimental Kd = 210 nM).
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Research and compare the top solutions currently on the market.
Once all parameters are set, users can start the simulation and observe in real time the display of the selected number of top solutions as the simulation progresses (Supplementary Fig. S2).
The lysate was centrifuged for 10 min at 13,000 ×g to separate high molecular weight, intact chromatin (pellet, designated as B) from cleaved, and low molecular weight DNA (top solution, designated as T).
Finally, 4 ml of a 5% sucrose/MBS solution were added as the top layer of the gradient.
Now, from the two populations of previous and current generation, the method selects the top ranked solutions as the participants for the next generation.
Noteworthy, the rankings obtained from the desirability and the utility functions had exactly the same sorting for the top ten solutions, as reported in Table 5.
That way the solutions and innovation comes from the bottom up as well as the top down.
The top two solutions from three independent docking runs (six solutions total) were compared.
Nortz believes that there are better solutions to the problem of homelessness in New York, citing more government funding to build housing as the top priority.
Ashima, as the top qualifier, went last.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com