Sentence examples for as the starting sample from inspiring English sources

A total of 50 ng of mRNA was used as the starting sample for the RNA amplification process using the WT-Ovation Pico System (NuGEN #3300) to generate 3 6 μg of amplified cDNA.

For example, when using only 10 μg of the four standard glycosylated-protein mixtures and 400 μg of bovine serum album interference as the starting sample, 42% of the annotated glycosites could still be quantified., higher than the convetional method of 26% [ 63].

DNA was extracted from all tissues using the QIAamp DNA Mini Kit (Qiagen, Valencia, California, USA) according to manufacturer's directions with the following exceptions: 80-120 mg of tissue was used as the starting sample and the sample was incubated at 56°C in proteinase K overnight for 16-18 hours.

Three of the methods used total RNA extracted from whole blood as the starting sample for mRNA amplification and target labeling: Affymetrix one-cycle target labeling (Method 1: no depletion_Affymetrix); Affymetrix one-cycle target labeling with globin PNAs added during cDNA synthesis (Method 2: Globin PNAs_Affymetrix); and NuGEN Ovation system v1 (Method 4: no depletion_NuGEN).

The properties of the adopted preamble's auto-correlation induce the formation of a plateau to M t c, whose length is equal to L - l, with l number of channel taps (excluding the line-of-sight component) as defined in Section 2. The starting sample of the preamble may be taken to be anywhere within this plateau [21], leading to some uncertainty for the receiver.

The values represent the relative activity recovered in each fraction with the initial activity in the starting sample taken as 100%.

Saccade direction was measured as the angle between start and end position samples, and saccades were related to the stimulus rotation cycle by noting which frame was on screen during the starting sample of the saccade.

When researchers use such pre-enriched samples as their starting sample for liquid chromatography/mass spectrometry (LC/MS), they "can get much, much deeper into these samples," says Carr. "It's very common now to get 20,000 30,000 phosphorylation sites identified, which is really quite deep".

While this protocol was optimized for HBEC, any mammalian cell type can be used as a starting sample for EMV isolation.

The system was allowed to equilibrate for 1 h prior to starting sample collection as per consensus guidelines (Bellander et al., 2004).

For this, AS-ATN, a parasite clone previously selected for limited artesunate (ATN) resistance [28], was used as the starting biological sample and subjected to prolonged and increasing exposure to the two drugs over many generations.