The serum leptin levels as assessed by two different immunoassays were very low in all three patients (1.6, 0.9, and 1.1 ng/ml by the first assay as well as 1.3, 1.2, and 1.1 ng/ml by the second assay, respectively) [15].
The second assay (Back Assay as it leads to NADPH formation) is based on the FabG-catalyzed oxidation of 3-OH decanoyl-NAC with NADP+ as cosubstrate.
The second assay using ascorbate as reducing agent provides a tool to measure PMO activity independently from the interaction between PMO and its electron donor CDH, but it is too prone to interference to be used in crude enzyme samples such as fermentation media.
Since all assays have sensitivity of 100%, in the event of shortage of any of the three assays, any available assay (among the three) can be used as the first assay.
In the second assay, fibrin clots, formed as described above, were perfused with the same buffer containing 0.2 μmol L-1 rt-PA according to Collet et al. [ 28].
The second assay detected Δ7Smn transcripts.
VL values measured as <400/cc by the first assay were assigned a value of 200/cc and all the VL values were log-transformed prior to analysis.
There was also a similar trend towards inbred females living shorter and inbred males living longer, as seen in the first assay.
