Sentence examples for as the initial template from inspiring English sources

The method begins with a protein-resistant surface containing a low percentage (1 5%) of surface-bound biotin on gold as the initial template.

Each PCR contained 30 ng of the pPhaCAB plasmid as the initial template, 250 ng of each primer, 200 μM of each dNTP, and 5 U of Taq DNA polymerase in Taq DNA polymerase reaction buffer.

After failed attempts to obtain X-ray crystallographic structures of the proteins investigated, homology models using 2vep structure from Streptomyces coelicolor [ 28] as the initial template were built for CAM1 (261 residues), CAM2 (203 residues) and CAM1_204 (204 residues).

This model includes a switch of the RT complex with nascent cDNA from RNA serving as the initial template for the reverse transcription to another RNA corresponding to the 5' part of the chimera, followed by the chimera integration into the host genome [ 3].

To investigate the presence of possible heterogeneity in Pol α, the average model obtained after refinement with EMAN (Ludtke et al, 1999) was used as the initial template for 3D maximum-likelihood (ML3D) classification (Scheres et al, 2007), to split the images into more homogeneous subsets of the data.

R-GECO1 and R-GECO1.2 in pTorPE [ 5] were used as the initial templates for engineering low-affinity red fluorescent genetically encoded Ca2+ indicators for optical imaging (LAR-GECOs).

The interview guide and findings presented in a previous study [ 11] served as basis for the initial template consisting of two higher-order themes: 1) "Strenuous work situation for HCWs" and 2) "Economy"; themes initially indicated to be characterized by inter-level discrepancies.

This allowed us to account for any variability in the initial template concentration as well as the conversion efficiency of the reverse transcription reaction.

Sensitivity was calculated for all tick samples as well as tick samples divided into gltA copy numbers in the initial template.

For bootstrap t-tests comparing the 5'∶3' ratio of ESCs with ESMVs, the initial template values were normalized as described above.

It also had to have an atomic structure similar to silicon's: semiconductors are grown as crystals in a vacuum chamber, and the substrate provides the initial template.