As with the initial experiments in [ 3], when SM ≥ 80% the ISL and wet-lab profiles are designated experimentally indistinguishable.
However, MDR1 expression did not increase as much as in the initial experiment.
This value is higher than any value obtained in the initial experiments as well as in the previously reported studies.
As expected from the initial experiments, treatment with BIM or C3 resulted in altered cell morphologies.
Heating not only leads to peak narrowing and a decrease in peak separation but, when a protein film at elevated temperature is returned to the cool temperature (283 K), broadening and peak separation occurs again, and although the signal intensity is depleted the peak width and peak separation are nearly identical upon the return to 283 K, as compared to the initial experiments.
We thank Candace Fanale, Lila Lopez-Vaquero, and Jhonatan Paredes for assistance in taking the photographs as well as running the initial experiments to rate the attractiveness of those photographs.
The standard deviations for the initial experiments, as shown in the three curves of Figure 2B, are 0.0750, 0.1209 and 0.1580 for the low, medium and high luminance cases respectively.
Most of the initial experiments were one-offs.
JF participated in the initial experiments.
As in the initial experiment, MZ1 proved the most active compound, with more than 90% of all BET proteins being removed at compound concentration down to 1 μM.
The reproducibility of the MOL-PCR assay was proven by an independent repeat of the subtyping method, in which all isolates of the validation panel and of the 2 outbreaks received the same MOL-PCR profile as in the initial experiment.
