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15 These pronounced spectral differences suggest that Λ,Λ-B binds to [d CGCGAATTCGCG ]2 in a similar way as the initial binding to ct-DNA.
These co-crystallized conformations of ligands were set as the initial binding conformations for LIE simulation.
The findings that partial dehydration occurred as the initial binding took place suggest that the spaces for binding between the caps and G-tetrads cannot accommodate fully hydrated K+ ion and dehydration is a prerequisite for K+ binding.
CTCF may serve as the initial binding factor, defining a grid of potential insulation sites based on high specificity sequence motifs.
Similar(56)
As noted above, mutants affecting fidelity in response to both near- and non-cognate codons are likely to be due to altered affinity at the initial binding step.
As expected, △1 behaved similarity to WT while the △2 and △3 mutants did not proceed past the initial binding location (lanes 6 and 8).
For the three α2-3 trisaccharides, we found that the initial rate of HA binding is proportional to the concentration of glycolipid in the SLB, Figure 1B, as expected from the initial binding kinetics at low surface HA coverage (see kinetic analysis below).
Data were fit to a double exponential equation to obtain an association rate constant k obs, with k obs = k 1, as this corresponds to the initial binding event between the protein and MANT-ATP.
Also recently, Du et al. demonstrated that cell surface vimentin serves as an attachment site that mediated the initial binding and subsequently increased the infectivity of EV71.
As the scaled population size decreases, the initial binding affinity of the common ancestor is on average smaller and so fewer substitutions are required between a pair of divergent lineages for an incompatibility to arise in a hybrid.
Furthermore, when response is mediated by a cascade of messengers following the initial binding of the ligand to the receptor, as long as the subsequent responses are the result of the messenger molecule binding to a single binding site, according to the law of mass action, the dose response curve will be the same sigmoid shape as the initial receptor binding dose response.
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