Sentence examples for as the first template from inspiring English sources

The a th fingerprint that the AVE a (F a ) is the maximum will be chosen as the first template fingerprint.

When uridine 5′-monophosphate (UMP), the most weakly pairing nucleotide, 32 was offered to a primer on a template with adenine as the first templating base, 75%% incorporation occurred after 2 d.

and the F c is as the third template.

The polystyrene beads trapped in the first template act as the second template, and cobalt nickel alloy is electrochemically grown through the voids between the beads.

Step 2. For the second template fingerprint, the fingerprint F b that the S(F a, F b ) is minimum will be chosen as the second template.

We selected 3MK7c as the second template on the basis of bioinformatic analysis.

With mRNA as the template, first-strand cDNA was synthesized by adding random hexamers and second-strand cDNA was synthesized by adding buffer, dNTPs, DNA polymerase I and RNase H. Double-stranded cDNA was purified using AMPure XP beads.

A final set of registrations were performed with the second reference template as the target (Fig. 2).

Next, overlapping PCR was performed with primer pair 5gΔTMF1/5gΔTMR2 using the mixture of the first and the second fragments as the PCR template.

1/V5-His-TOPO vector (Invitrogen) with High Fidelity PCR Enzyme (Fermentas) using 1 µl of the first strand as template.

The Gateway® cloning system uses a nested PCR method involving two rounds of amplification in which the second round uses the product of the first round as template.