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A solar panel on the International Space Station tore yesterday as the array was unfurled, presenting a new challenge for a space shuttle mission that has already had more than its share.
As the array was designed from ESTs before a reference tomato genome sequence was available, for each differentially expressed probe, a similarity analysis was conducted with blastN against SGN Tomato Unigene database (http://solgenomics.net/).net/
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Also beta-glucuronidase/ GUSB (H01), which was referred to as the housekeeping gene in the array, was significantly downregulated.
The Array was built as a joint project between the SETI Institute (my employer) and the University of California at Berkeley's Radio Astronomy Laboratory.
The background of the array was used as the control to determine the actual presence of a protein in the (urine) sample.
If the percentage exceeded 15%, the array was discarded as an outlier array.
Following hybridization, each locus on the array was identified as N. vitripennis, N. giraulti, or ambiguous.
Hybridisation of cell line and control DNA to the array was performed as described [ 24].
Presence/absence for the array was determined as described below in data analysis.
Using the arbitrary 2-fold cutoff about 4.2% of the probesets on the array was identified as heat-stress responsive.
The complete list of genes in the array was used as a reference set in the analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com