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Sequence alignments are available as supplementary Text S1 and S2.
Statistical analysis was performed using the stats package in R version 2.1[37]; the code is included as Supplementary Text S3.
Cx50 shares 52% sequence similarity with Cx26 (multiple sequence alignment provided as Supplementary Text in Supplementary Material available online at http://dx.doi.org/10.1155/2014/673895).org/10.1155/2014/673895
In silico analyses revealed that all of the functionally characterized human Cx50 missense mutations alter highly conserved residues (Supplemental Figure 1; multiple sequence alignment provided as supplementary text).
As Supplementary Text 1 demonstrates, the results hold if empirical values are used where they are known, choosing appropriate values for the remaining free parameters.
This quotient has its maximum of 1, which occurs if both sequences are equal over the length of the shorter sequence and its minimum of 0, if the alignment score for the sequence pair is 0. A detailed description of the combination approach, the SeedMatch algorithm and the parameters used is available as supplementary text (see Additional file 5).
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The works not admitted to the canons (those of a semisacred or semicanonical character) may still be quite valuable as supplementary texts.
All the technical aspects that are not most essential in understanding the basic idea are provided either as supplementary texts or supplementary figures.
Receptor phosphorylation can also be affected by the organization of GPCRs on the membrane as well (see Supplementary Text S2 and Figure S5).
Basophil activation was measured using rat basophilic leukaemia cells that expressed the human FcεRI as described in Supplementary Text S2.
As a result, this method groups multiple non-identical domain combinations as one (Supplementary Text S2), which reduces the total number of types of combinations, moves the predicted evolutionary origin of each domain combination towards the root of the tree, and in some case increases the number of independent genesis events of domain combinations.
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CEO of Professional Science Editing for Scientists @ prosciediting.com