The phrase "as suggested in the protocol" is correct and usable in written English.
You can use it when referring to recommendations or guidelines outlined in a specific protocol or set of instructions.
Example: "The experiment should be conducted as suggested in the protocol to ensure accurate results."
Alternatives: "as indicated in the protocol" or "as outlined in the protocol".
Incubation of membranes with secondary antibodies was done at room temperature for 1 hr, and proteins detected by enhanced chemiluminescence reagents (Pierce), as suggested in the manufacturers protocol.
Starting primer pairs were designed as suggested in the AMBION protocol and according to the guidelines published in [25].
Additionally, centrifugation steps of Qiagen spin columns were done at 8000 rpm instead of 13000 rpm as suggested in the Qiagen protocol.
Sudan Black B staining for lipofuscin was performed as described [ 28] with some modifications: to achieve sufficient staining, paraformaldehyde-fixed fibers [ 27] were stained for two hours (rather than 2 to 8 minutes as suggested in the original protocol) with 0.7% Sudan Black B dissolved in 70% ethanol.
Small simplifications to questionnaires were made; participants would be able to indicate their preference for a health state by circling it, whereas in the pilot study participants used ticks and crosses to indicate a preference as suggested in the MVH protocol (MVH Group, [ 20 ]).
For the mouse samples, miRNAs were considered expressed in a given tissue when a minimum of 3 out of the 4 samples analysed had CT values below 32, as suggested in the manufacturer's protocol.
Patients with HIV/VL coinfection were excluded from the study as these treatments are not recommended for this group [ 5]; however, their data were recorded in the trial surveillance register and as suggested in the pilot study protocol were treated on a compassionate basis with a combination regimen of AmBisome and miltefosine.
The optimum numbers of PCR cycles, checked as suggested in the SMART-PCR kit protocol, were 22 (rab1), 20 (bov1 and rab2), 23 (respectivelyctively.
In experiment 9, an amount of 2500 microspheres of each region per reaction in combination with a concentration of 10 μg/mL of SAPE in the reporter mix was taken as reference condition, as suggested in the Luminex no-wash protocol [ 1].
