A proportion of the other cases may not have been identified at the point of diagnosis as study isolates because incomplete demographic information was supplied with the sample.
As a control in this study, isolate 1320 was digested and underwent electrophoresis five independent times, and each time yielded the same PFGE pattern.
These limitations make ground-based approaches viable for studying isolated features, as opposed to landscape-scale phenomena.
We selected for inclusion in this study isolates identified as single-genome infections by genotyping of Merozoite Surface Protein 2 (msp2) [28].
In the current study, isolates reported as being non-susceptible to tigecycline by the Vitek2 system were confirmed with the E-test method to avoid the misclassification of the results.
Traditionally molecular biology research has tended to reduce biological pathways to composite units studied as isolated parts of the cellular system.
Though Banks suggests that PACAP has actions in the CNS and provides evidence in support [95], the available data strongly indicate that PACAP does not pass the wall of cerebral arteries, as studied in isolated perfused MCAs [96], which agrees with the view that the peptide is a large molecule that does not pass the blood-brain barrier (BBB) [96, 97].
In the present study we examined whether the acute stimulation of GSIS by GLP-1 or Ex-4 involves the modulation of glucose, fatty acid and energy metabolism in the β-cells, as studied using isolated islets of both rats and mice.
Therefore further studies are required, using primary VSMCs cultured in different glucose media, as well as studying cells isolated from normal and severely diabetic animals, in order to corroborate this hypothesis.
Through minimization of confounding variables relating to pathology, surgeon, institution, soft-tissue balancing, and type of prosthesis, this study isolates articular design as a variable.
