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Both differentiated and undifferentiated stem cells have been used as starting cell sources.
The cell number at the time point of 6 hr was used as starting cell number (N0).
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This population of NPCs was used as starting cells for iPS induction in the following experiments.
In this study, we describe a defined, serum-free two-step differentiation system using both human ESCs and iPSCs as starting cells.
ESCs from the morula were chosen as the starting cell material for cell therapy of diseased retina as they are totipotent, that is able to differentiate into any adult cell type, and have potentially unlimited self-renewal capacity.
MCF-10AT cells were originally chosen as the starting cell population for our study because of the intrinsic low incidence of tumor formation [ 18– 20] and the lack of any cancer stem cell-like characteristics.
Although a well-established cell type in many fields of research, due to their ease of handling and the cost-effectiveness, there are disadvantages to the use of fibroblasts as a starting cell type for producing hiPSCs.
The sensitivity of strains lacking components of these HAT complexes has been validated on agar plates using several starting cell densities as well as three different concentrations of CG-1521.
HeLa cells were seeded at a density of about 20% confluence (designated as a relative starting cell number of 100%) and then incubated at these curcuminoid concentrations for three days.
The percentage of ghost cells decreases with an increase in the starting cell as shown in Figure 6, and the error decreases with increase in the total number of cells as shown in Figure 7.
Cells were then distributed to Bioscreen C microplates containing media as described to a starting cell density of OD600nm = 0.05.
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