By using the ten WHO Population Screening Principles [ 28] as a framework, we developed the principles of clinical interventions focused on QoL assessment in palliative care, as specified in the following procedures.
Single genome amplification and sequencing was performed as specified in the Supplemental Experimental Procedures.
Virus stocks were generated and used for transduction of various cell lines as specified in the Supplemental Experimental Procedures.
Statistical analyses were made using either Student's two-tailed unpaired t test or analysis of variance (ANOVA) as specified in the figure legends, and Newman-Keuls procedure was used for multiple comparison analysis.
As specified in the study design, a curative surgical tumour resection procedure was performed in 17 out of 44 patients (PP analysis): two in the Con arm and 15 in the PET arm (Table 2).
For each study, one member of the evaluation team performs the data extraction, and quality assurance procedures are undertaken as specified in the protocol (e.g., review and confirmation by another team member).
The program on the experimenter's side, psycon.exe, administers the entire procedure: presenting the stimuli as specified in the AUX script, collecting the subject's response, providing feedback if desired, and displaying and saving the result upon completion of the session.
In all experiments, the absorbance of the products of the kit reactions was measured at 540 nm, as specified in the instructions for the Sigma kit, even though the StanBio kit procedure calls for measurement at 500 nm.
Total RNA was prepared using the acid phenol-guanidinium thiocyanate procedure [ 65] with commercial TRIzol® Reagent (Invitrogen Life Technologies) as specified in the manufacturer's protocol.
Data were statistically analyzed using either the Student's t test for unpaired or paired values depending on experimental procedures, or a two-way ANOVA followed by a Bonferroni post-test, as specified in the legends.
