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Confocal images (stacks) were acquired at 0.2 micron spacing with an Olympus 100× or a 40× Oil immersion objective as specified in the figure legends with an Applied Precision DeltaVision RT microscope system (Applied Precision, Issaquah, WA).
For all studies it was verified whether the reported laboratory parameters were in adherence to the objectives of the study as specified in the paper.
Timelapse start and duration was as specified in the experiment.
The system works exactly as specified in the specification.
The title will transfer as specified in the agreement.
as specified in the Results section.
or ±standard deviation (SD) as specified in the figure legends.
Antibody dilutions were used as specified in the accompanying datasheet.
as specified in the figure legends.
Analysis parameters used were as specified in the dChip manual.
These items were assessed as specified in the study protocol.
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