Initial uncertainty regarding parameter values is expressed in the form of vague/noninformative prior distributions, as specified in the Methods.
Western blotting was carried out using standard protocols [ 19] and tissue microarray resourced immunohistochemistry analysis and functional assays were performed as specified in the Methods section.
1c – as specified in the methods section we maintained cells in DMEM from Invitrogen (#10569-010).
The membership of breeds to four groups, as specified in the Methods section, is also indicated.
Log2-transformations of this normalization were performed as specified in the methods below.
These modifications are all synonymous substitutions occurring only at nucleotide level (as specified in the Methods ).
Of this study new data were requested and received, using the matching algorithm as specified in the methods section.
After applying quality filters as specified in the methods section, 227,892 (98.4%) sequences passed the initial filters.
Following fragmentation the manual library preparations were performed as specified in the standard protocol, excluding the second agarose gel separation.
The remaining reagents (buffer, dNTPs, dithiothreitol, RNase inhibitor, Thermoscript] were added as specified in the Thermoscript protocol.
These items were assessed as specified in the study protocol.
