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In order to examine volume-sensitive amino acid release in vivo, rat cortices were perfused via a microdialysis probe with hypoosmotic medium in which [NaCl] was reduced from 120 to 25 mM NaCl (65% reduction in osmolarity; medium also contained 25 mM NaHCO3 and other salts as specified in the Methods section).
SVM-based classification models were built utilizing a small pool of topological descriptors as specified in the Methods section.
Initial uncertainty regarding parameter values is expressed in the form of vague/noninformative prior distributions, as specified in the Methods.
1c – as specified in the methods section we maintained cells in DMEM from Invitrogen (#10569-010).
The membership of breeds to four groups, as specified in the Methods section, is also indicated.
Log2-transformations of this normalization were performed as specified in the methods below.
These modifications are all synonymous substitutions occurring only at nucleotide level (as specified in the Methods ).
Of this study new data were requested and received, using the matching algorithm as specified in the methods section.
After applying quality filters as specified in the methods section, 227,892 (98.4%) sequences passed the initial filters.
SK-BR-3 and BT-474 cells were used as target cells and the ADCC assay was performed as specified in the Methods section.
Using the Sputnik program and threshold values as specified in the Methods section, we searched for potential microsatellite (SSR) sequences in the 3716-candidate gene set.
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