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In all experiments, the absorbance of the products of the kit reactions was measured at 540 nm, as specified in the instructions for the Sigma kit, even though the StanBio kit procedure calls for measurement at 500 nm.
Leave the dye on as specified in the instructions for your dye.
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The siRNAs were transfected through electroporation, as specified in the instruction manual (Amaxa, Germany).
All tests used an LC Plus nebulizer which was filled with 3 mL inhalation solution and operated as specified in the instruction manual to deliver aerosol for 10 min into the impactor circuit.
Lysis solution (DNeasy® kit buffer AP1 containing 25 mg mL−1 polyvinylpolypyrrolidone), along with RNase A, was added to the powdered leaf samples and the mixture was incubated at 65 °C, as specified in the kit instructions.
at 30 Hz for 1 min. Lysis solution (DNeasy® kit buffer AP1 containing 25 mg mL−1 polyvinylpolypyrrolidone), along with ribonuclease I, was added to the powdered leaf samples and the mixture was incubated at 65 °C, as specified in the kit instructions.
The PanBio Units reported represent the ratio of sample ODs to a cut-off value, as specified in the manufacturer's instructions.
Protein digests were performed as specified in the manufacturer's instructions for the Thermo Scientific's TMTsixplex Isobaric Mass Tagging Kit (catalog #90064B).
The multiplex assay used Beadmate kits (Upstate) as specified in the manufacturer's instructions, read with a Luminex 100 system (Upstate).
750 ng amplified cRNA was labeled and hybridized to Illumina Human HT-12 v4 beadchips as specified in the manufacturer's instructions.
When needed and for comparative purpose, RNA was extracted from the same tissue samples and from the vaccine virus, Nigeria 75 1 strain, using QIAGEN RNeasy Mini Kit as specified in the manufacturer's instructions.
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